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Berman, Helen M.

Position: 
Board of Governors Professor of Chemistry and Chemical Biology

Helen M. Berman Phone: 848-445-4667
E-mail: E-mail
FAX: 732-445-4320
Office: Center for Integrative Proteomics Research (CIPR) 117
Mail: SAS - Chemistry & Chemical Biology
174 Frelinghuysen Rd
Piscataway, NJ 08854-8076
Education Links
  • A.B., 1964, Barnard College
  • Ph. D., 1967, University of Pittsburgh

Research Summary

As a founding member of the Worldwide Protein Data Bank (wwPDB) collaboration, we support scientific research and education by providing the essential Protein Data Bank (PDB) archive of information about the experimentally-determined structures of proteins, nucleic acids, and complex assemblies. The wwPDB organization ensures that the PDB is freely and publicly available to the global community. Members host deposition, annotation, and distribution centers for PDB data and collaborate on a variety of projects and outreach efforts, including developing tools and processes for validating and remediating structural data.

The Research Collaboratory for Structural Bioinformatics (RCSB) PDB, a collaboration between Rutgers and the University of California, San Diego, RCSB PDB maintains the central repository of the PDB archive for the wwPDB, and provides resources that promote a structural view of biology, including a relational database, molecular viewers, structure comparison tools, and educational materials such as the Molecule of the Month series.

EMDataBank.org is a joint effort of the Protein Data Bank in Europe, the RCSB, and National Center for Macromolecular Imaging that provides a global deposition and retrieval network for cryoEM map, model and associated metadata, and a portal for software tools for standardized map format conversion, map, segmentation and model assessment, visualization, and data integration.

With the overarching goal of creating new knowledge about the interrelationships of sequence, structure and function, the Structural Biology Knowledgebase (SBKB) creates an information repository and web portal that highlights Protein Structure Initiative and broader structural biology research activities. It combines methodological, structural, and functional data in a single, easily-searchable website, and encourages collaborative interactions between the PSI and the biological communities.

The work on these projects has been informed by our research and studies in structural biology, in particular protein-nucleic acid interactions, binary and ternary complexes with catabolite activating protein (CAP), and nucleic acids. Several studies have been focused on defining the precise molecular interactions involved in transcription activation by CAP. In a project led by Catherine Lawson, we obtained an experimental 3D map volume of a class I CAP-dependent transcription activation complex using single particle averaging of 2D electron microscopy images. The EM map volume and fitted coordinates are deposited in the EM Databank (EMD-5127) and PDB (id 3iyd).

Three-dimensional EM structureThree-dimensional EM structure of an intact activator-dependent transcription initiation complex. Electron microscopy reconstruction (20 Å resolution) of a transcription initiation complex from Escherichia coli comprising RNA polymerase holoenzyme (purple with alpha subunit C-terminal domain shown in green), catabolite activator protein (blue), and DNA (tan). The background is an electron micrograph of reconstituted complex particles in negative stain (Hudson et al., 2009).

 

 

 

 

 


Awards & Honors

  • Board of Governors Professor of Chemistry and Chemical Biology
  • Fellow, American Association for the Advancement of Science
  • Fellow of the Biophysical Society
  • 2000 Distinguished Service Award, Biophysical Society
  • 2006 M.J. Buerger Award
  • 2007-2009 Distinguished Lecturer, Sigma Xi
  • 2010 Department of Chemistry Alumni Award, University of Pittsburgh
  • Fellow, American Crystallographic Association
  • 2012 Carl Brändén Award, Protein Society
  • 2013 DeLano Award for Computational Biosciences, American Society for Biochemistry and Molecular Biology

Selected References

Hudson BP, Quispe J, Lara-González S, Kim Y, Berman HM, Arnold E, Ebright RH, Lawson CL. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):19830-5. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775702/)

Berman HM, Westbrook JD, Gabanyi MJ, Tao W, Shah R, Kouranov A, Schwede T, Arnold K, Kiefer F, Bordoli L, Kopp J, Podvinec M, Adams PD, Carter LG, Minor W, Nair R, La Baer J. The protein structure initiative structural genomics knowledgebase. Nucleic Acids Res. 2009 Jan;37(Database issue):D365-8. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2686438)

Berman H, Henrick K, Nakamura H. Announcing the worldwide Protein Data Bank. Nat Struct Biol. 2003 Dec;10(12):980.

Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE. The Protein Data Bank. Nucleic Acids Res. 2000 Jan 1;28(1):235-42. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC102472)

Schneider B, Berman HM. Hydration of the DNA bases is local. Biophys J. 1995 Dec;69(6):2661-9. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1236503)

Bella J, Eaton M, Brodsky B, Berman HM. Crystal and molecular structure of a collagen-like peptide at 1.9 Å resolution. Science 1994 266:75-81. (http://www.ncbi.nlm.nih.gov/pubmed/7695699)

Berman HM, Olson WK, Beveridge DL, Westbrook J, Gelbin A, Demeny T, Hsieh SH, Srinivasan AR, Schneider B. The nucleic acid database. A comprehensive relational database of three-dimensional structures of nucleic acids. Biophys J. 1992 Sep;63(3):751-9. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1262208)


Research Areas: 
Biophysical Chemistry

Rutgers, The State University of New Jersey