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	KiBum Lee Assistant Professor Email
	Contact   Links Phone: (732) 445-2081 Fax: (732) 445-5312 Lab: (732) 445-???? Dept: (732) 445-2618 Office: WL-315 Mail: Chemistry & Chemical Biology, 610 Taylor Road, Piscataway, NJ 08854 IGERT Graduate Fellowship Program Institute for Advanced Materials, Device and Nanotechnology The Rutgers Stem Cell Research Center

The primary research interest of our group is to develop and integrate nanotechnologies and chemical functional genomics to modulate signal pathways in cells (e.g. stem cells and cancer cells) towards specific cell lineages or stages. We also intend to probe biological behaviors (e.g. phosphorylation and cell adhesion) of dynamic cell processes, such as proliferation, differentiation, and migration, as shown in Figure 1. The methodologies developed in our group will be widely applicable to the study of other cellular processes, including cell development, neurogenesis, and intracellular tracking. Thus, our research program is directly relevant to matters concerning biomaterials, nanomedicine, chemical biology and stem/cancer cell biology.

Development of novel bionanotechnology methods to probe biomolecular interactions of cells in vivo and in vitro
The elucidation and regulation of molecular interaction mechanisms through which biomolecules (e.g. proteins, peptides, synthetic small molecules, and siRNA etc.) bind to their targets in specific signaling cascades is crucial to the advancement of cell biology. For this purpose, our first research project focuses on two novel methods in bionanotechnology and cell biology: (i) synthesizing multifunctional nanocomposites (Figure 2.) and modifying their surface with biomolecules (e.g. signaling molecules and siRNA etc.) for investigating biomolecular interactions within cellular compartments and (ii) generating combinatorial biomolecular nanoarrays and analytical tools to allow scientists to study and deliberately control interactions at the subcellular and single molecule levels. Nanostructures and nanomaterials intrinsically can interact with biological systems at a fundamental, molecular level with high specificity and selectivity. The key question is how can we take advantage of those unique molecular properties in orderto detect biomolecular interactions and dissect cellular signaling pathways at the single molecular level. In order to address these challenges, I propose to develop and integrate nanotechnologies to generate novel methodology platforms for identifying and regulating subcellular interactions.

Combinatorial biomolecular nanoarrays for probing biological interactions
The goal of this project is to apply combinatorial biomolecular nanoarrays and analytical tools to systematically study vital questions in molecular biology and cell biology (Figure 3.). While it is important to dissect and modulate cellular signaling pathways using chemical genomic and proteomic approaches, it is also crucial to better elucidate stem cell interactions with microenvironments at the single molecular level. To achieve this specific aim, we will focus on elucidating interactions between stem cell (and cancer cell) and extracellular microenvironments (or surface ligands) at the molecular level. Some of the critical questions that will be addressed through this project include: i) how cells respond to anisotropic physical (e.g. pattern shapes, sizes, and morphologies) and chemical cues (e.g. compositions) in nanoscale spatial organization, where cells self-renew, differentiate, migrate, interact with one another, and transduce signals as a function of external stimuli in the context of predetermined surface patterns; and ii) how does a patterned surface (two or three-dimensional) provide signal information regarding the presence of a particular biological entity or itself induces a desirable cellular phenotype. These new combinatorial nanoarrays and analytical tools should facilitate biomedical research and cell biology (e.g. stem cell biology or cancer cell biology) by offering the capability to customize a surface on the 1-100 nm length scale and/or to detect extraordinarily rare analytes in complex environments.

Regulating Stem cell fate: chemical biology and nanotechnology approaches to elucidate and modulate the signaling pathways in stem cells
The goal of this project is to develop and integrate chemical functional genomics and nanotechnologies for the dissection and modulation of the signaling pathways involved in the differentiation of stem cells towards specific cell lineages. Furthermore, we propose to probe biological behaviors (e.g. phosphorylation and stem cell adhesion) of dynamic stem cell self-renewal and differentiation processes. The proposed research will combine chemical functional genomic tools and quantitative phospho-proteomic analysis with biochemical methods (e.g. affinity chromatography) and functional genomic tools (e.g. siRNA knockdown and cDNA complementation). In addition, to gain an understanding of molecular mechanisms by which chemical cues (e.g. growth factors and ligands etc.) bind to their targets, we will use nanoprobes (e.g. magnetic nanoparticles or magnetic quantum dots) to trace the interactions of biomolecules with their stem cell receptors in vivo and/or in vitro. The methodologies developed in this proposal will be widely applicable to the study of other cellular processes, including stem cell development, cell migration, neurogenesis and intra-cellular trafficking. Thus, this project is directly relevant to chemical biology, nano medicine and stem cell biology. The following specific aims will address the aforementioned problems and limitations.

• B.S. 1998, Kyung Hee University (Seoul, Korea)
• M.S. 2000, KAIST (Taejon, Korea)
• Ph.D. 2004, Northwestern University (Evanston, IL)
• Postdoctoral Research Fellow 2004-2007, The Scrrips Research Institute

• CIRM (California Institute for Regenerative Medicine) Post-doctoral Fellowship (2006-2007)
• NSEC (Nanoscale Science and Engineering Center) Outstanding Research Award (2004)
• MRS Graduate Student Award (2003)
• L. Carroll King Award, Northwestern University (2001)
• University Fellowship, Northwestern University (2001-2002)

1. Ivanisevic, A.; Im, J.-H.; Lee, K. -B.; Park, S.-J.; Demers, L. M.; Watson, K. J.; Mirkin, C. A., "Redox-Controlled Orthogonal Assembly of Charged Nanostructures", J. Am. Chem. Soc., 2001, 123, 12424-12425.
2. Lee, K. -B.; Park, S. -J.; Mirkin, C. A.; Smith, J. C.; Mrksich, M., "Protein Nanoarrays Generated by Dip-Pen Nanolithography", Science, 2002, 295, 1702-1705.
3. Lee, K. -B.; Lim, J. -H.; Mirkin, C. A., "Protein Nanostructures Formed Via Direct-Write Dip-Pen Nanolithography", J. Am. Chem. Soc.; 2003, 125, 5588-5589.
4. Lim, J. -H.; Ginger, D.; Lee, K. -B.; Heo, J.; Nam, J. -M.; Mirkin, C. A., "Direct-Write Dip-Pen Nanolithography of Proteins on Modified Silicon Oxide Surfaces", Angew. Chem. Int. Ed., 2003, 20, 2411-2414.
5. Smith, J. C. *; Lee, K. -B.*; Wang, Q. *; Finn, M. G.; Johnson, J. E.; Mrksich, M.; Mirkin, C. A., "Nanopatterning the Chemospecific Immobilization of Cowpea Mosaic Virus Capsid", Nano Letters, 2003, 3, 883-886. (* These authors contributed equally to this work.)
6. Zhang, H.; Lee, K. -B.; Li, Z.; Mirkin, C. A., "Biofunctionalized nanoarrays of inorganic structures prepared by dip-pen nanolithography", Nanotechnology, 2003, 14, 1113–1117.
7. Nam, J. -M.; Han, S. W.; Lee, K. -B.; Liu, X.; Mirkin, C. A., "Bioactive Protein Nanoarrays on Nickel Oxide Surfaces Formed by Dip-Pen Nanolithography" , Angew. Chem. Int. Ed., 2004, 43, 2146-1249.
8. Zhang, Y.; Salaita, K.; Lim, J -H.; Lee, K. -B.; Mirkin, C. A., "A Massively Parallel Electrochemical Approach to the Miniaturization of Organic Micro- and Nanostructures on Surfaces", Langmuir, 2004, 20, 962-968.
9. Lee, K. -B.*; Park, S.*; Mirkin, C. A., "Multicomponent Magnetic Nanorods for Biomolecular Separations", Angew. Chem. Int. Ed. 2004, 43, 3048-3050. (* Equal contribution)
10. Lee, K. -B.; Kim, E. -Y.; Wolinsky, S. M.; Mirkin, C. A., "The use of nanoarrays for highly sensitive and selective detection of human immunodeficiency virus in plasma", Nano Letters, 2004, 4, 1869-1872.
11. Oh, B.-K; Park, S.; Millstone, J. E.; Lee, S. W.; Lee, K. -B.; Mirkin, C. A., "Separation of Tricomponent Protein Mixtures with Triblock Nanorods", J. Am. Chem. Soc., 2006, 128, 11825-11829.