Dr. K.Y. Chen's Laboratory - Hypusine and eIF5A

Interacting Partners of eIF5A

What are the eIF5A interacting proteins?

What are the eiF5A interacting proteins

What has been done?

done

Construction of the TIF51A-TAP strain

construct

TAP Protocol

TAP Pro

TAP method: Results

TAP Method

Profile of TAP-eIF5Aa Binding Proteins

pro of TAPeiF5A

The TIF51A-binding partner is 80S ribosome

TIF51A binding partner 80S

TIF51A-ribosome interaction is sensitive to RNase and EDTA

TIF51A ribosome

Endogenous TIF51A co-sediments with ribosomes

Endogenous TIF51A2

80S ribosome is the interacting partner of eIF5A

80 Sribosomeis the interacting partner of eIF5A

Binding of eIF-5A to different populations of ribosomes

Binding of eIF5A to different populations of ribosomes

Binding of eIF-5Q to different populations of ribosomes

Binding of eIF5Q to different populations of ribosomes

Is the eIF-5A-80S ribosome binding hypusine-dependent?

Is the eIF5A 80S ribosome binding hypusine dependent

Stably transfected cells containing FLAG-tagged eIF5A

Stably transfected cells containing FLAG tagged eIF5A

Hypusine-dependent eIF-5A-ribosome interaction in mammalian cells

Hypousine dependent eIF5A ribosome interaction in mammalian cells

Diagram 1

Diagram 1

A temperature-sensitive mutant with eIF5A mutation

A temperature sensitive mutant withe IF5A mutation

Effect of eIF5A on cytoskeleton and actin dynamics