Dr. K.Y. Chen's Laboratory - Hypusine and eIF5A

Hypusine Publications

Chen, K.Y. and Liu, A.Y.-C. Differences in polyamine metabolism of the undifferentiated and differentiated neuroblastoma cells. Metabolic labeling of an 18,000-Mr protein by [14C]-putrescine and the conversion of putrescine to GABA. FEBS Letters, 134, 71-74, 1981.

Chen, K.Y. An 18,000-dalton protein metabolically labeled by polyamine in various mammalian cell lines. Biochim. Biophys. Acta, 756, 395-402,1983.

Chen, K.Y. and Dou, Q.P. NAD+ stimulated the spermidine-dependent hypusine formation on the 18,000-dalton protein in cytosolic lysates derived from NB-15 mouse neuroblastoma cells. FEBS Letters, 229, 325-328, 1988.

Chen, K.Y. and Dou, Q.P. Isolation and characterization of an 18,000-dalton hypusine-containing protein from NB-15 mouse neuroblastoma cells. Biochim. Biophys. Acta, 971, 21-28, 1988.

Dou, Q.P. and Chen, K.Y. Two hypusine-containing proteins identified by metabolic labeling in chick embryo fibroblasts. J. Chinese Chem. Soc., 36, 443-450, 1989.

Yang, Y.C., Chen, K.Y., Seyfzadeh, M. and Davis, R.H. Deoxyhypusine/hypusine formation on a 21,000-dalton cellular protein in a Neurospora crassa mutant in vitro and in vivo. Biochim. Biophys. Acta,1033, 133-138, 1990.

Dou, Q.P. and Chen, K.Y. Characterization and reconstitution of a cell free system for NAD+-dependent deoxyhypusine formation on the 18,000-dalton eIF-4D precursor. Biochim. Biophys. Acta, 1036, 128-137, 1990.

Knapp, S., Hale, J.J., Bastos, M., Molina, A. and Chen, K.Y. Synthesis of hypusine and other polyamines using dibenzyltriazones for amino protection. J. Org. Chem., 57, 6239-6256, 1992. J. Org. Chem 1992

Tao, Y., Skrenta, H.M. and Chen, K.Y. Deoxyhypusine synthase assay based on the use of polyhistidine-tagged substrate and metal chelate affinity chromatography. Anal. Biochem., 221, 103-108, 1994.  Anal. Biochem., 221, 103-108, 1994

Tao, Y. and Chen, K.Y. Purification of deoxyhypusine synthase from Neurospora crassa to homogeneity by substrate elution affinity chromatography. J. Biol. Chem., 270, 383-386, 1995.

Tao, Y. and Chen, K.Y. Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast, deoxyhypusine synthase cDNA. J. Biol. Chem., 270, 23984-23987, 1995.

Yan, Y.P., Tao, Y. and Chen, K.Y. Molecular cloning and functional expression of human deoxyhypusine synthase cDNAs based on expressed sequence tag information. Biochem. J., 315, 429-434, 1996.

Chen, Z.P., Yan, Y.P., Ding, Q., Potenza, J.A., Knapp, S., Schugar, H.J. Chen, K.Y. Effects of inhibitors of deoxyhypusine synthase on the differentiation of mouse neuroblastoma and ertthroleukemia cells. Cancer Letters, 105, 233-239, 1996.

Chen, K.Y. and Liu, A.Y.-C. Hypusine formation on eukaryotic initiation factor 5A, biochemistry and function. Biol. Signals, 6, 105-109, 1997.

Chen, Z.P and Chen K.Y. Dramatic attenuation of hypusine formation on eukaryotic initiation factor 5A during senescence of IMR-90 human diploid fibroblasts. J. Cell. Physiol., 170, 541-548, 1997.

Chen, Z.P. and Chen, K.Y. Marked elevation of hypusine formation on eukaryotic initiation factor 5A in v-Ha-ras transformed mouse NIH3T3 cells. Cancer Letters, 115, 235-241, 1997.

Liu, Y.P., Nemeroff, M., Yan, Y.P. and Chen, K.Y. Interaction of eukaryotic initiation factor 5A with human immunodeficiency type I virus Rev response element and U6 snRNA requires deoxyhypusine or hypusine modification. Biol. Signals, 6, 166-174, 1997.

Chen, K.Y. and Jao, D.L Chemistry of hypusine formation on eukaryotic initiation factor 5A,in biological systems J. Chinese Chemical Society, 146, 727-734, 1999.

Xu, A. and Chen, K.Y. Hypusine is required for a sequence-specific interaction of eukaryotic initiation factor 5A with post-SELEX RNA. J. Biol Chem., 276, 2555-2561, 2001.

Jao, D.L. and Chen, K.Y. Subcellular localization of the hypusine-containing eukaryotic initiation factor 5A by immunofluorescent staining and Green Fluorescent protein tagging. J. Cell. Biochem., 86, 590-600, 2002.

Xu, A.Jao, D.L. and Chen, K.Y. Identification of messenger RNA species that bind specifically to eukaryotic initiation factor 5A by affinity co-purification and differential display. Biochemical Journal, 384, 585-590, 2004.

Jao DL, Chen KY. Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex. J Cell Biochem. 97, 583-598, 2006.

Chatterjee I, Gross SR, Kinzy TG, Chen KY. Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression. Mol Genet Genomics. 275:264-276, 2006.