BEGIN:VCALENDAR VERSION:2.0 PRODID:-//jEvents 2.0 for Joomla//EN CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT UID:03a58941652114b5d263e8abd1ebffb3 CATEGORIES:Colloquium CREATED:20190819T133930 SUMMARY:Professor Benjamin Schuster DESCRIPTION:
Sequence determinants of protein phase separati on: Biophysical principles and synthetic biology applications
< p>Membraneless organelles are intracellular compartments with important bio logical functions, ranging from stress response to regulation of gene expre ssion. These compartments lack an enclosing membrane, instead forming via l iquid-liquid phase separation arising from multivalent interactions. Membra neless organelles are often enriched in intrinsically disordered proteins ( IDPs), which have been identified as key drivers of phase separation. In th is talk, I will first present our recent work to understand the sequence fe atures responsible for phase separation of a prototypical IDP, the arginine /glycine-rich RGG domain from LAF-1. We identified three key features respo nsible for phase separation of this RGG domain: a short conserved sequence, charge patterning, and arginine-tyrosine interactions. These findings are the result of a collaboration (with Jeetain Mittal, Lehigh University) comb ining experiments with predictive simulations. In the second part of my tal k, I will discuss our work on engineering protein phase separation to gener ate designer membraneless organelles for synthetic biology applications. We devised strategies for enzymatically triggering assembly and disassembly o f membraneless organelles, as well as approaches for recruiting specific ca rgo proteins into the synthetic organelles in living cells. Together, this work advances our knowledge of the sequence-to-phase behavior relationship of IDPs, important both for understanding the biochemistry of membraneless organelles, as well as for designing biomaterials and synthetic organelles.~ Coffee/tea will be s erved prior to lecture~
X-ALT-DESC;FMTTYPE=text/html:Sequence determinants of protein phase separation: Biophysical principles and synthetic biology a pplications
Membraneless organelles are intracellular compar tments with important biological functions, ranging from stress response to regulation of gene expression. These compartments lack an enclosing membra ne, instead forming via liquid-liquid phase separation arising from multiva lent interactions. Membraneless organelles are often enriched in intrinsica lly disordered proteins (IDPs), which have been identified as key drivers o f phase separation. In this talk, I will first present our recent work to u nderstand the sequence features responsible for phase separation of a proto typical IDP, the arginine/glycine-rich RGG domain from LAF-1. We identified three key features responsible for phase separation of this RGG domain: a short conserved sequence, charge patterning, and arginine-tyrosine interact ions. These findings are the result of a collaboration (with Jeetain Mittal , Lehigh University) combining experiments with predictive simulations. In the second part of my talk, I will discuss our work on engineering protein phase separation to generate designer membraneless organelles for synthetic biology applications. We devised strategies for enzymatically triggering a ssembly and disassembly of membraneless organelles, as well as approaches f or recruiting specific cargo proteins into the synthetic organelles in livi ng cells. Together, this work advances our knowledge of the sequence-to-pha se behavior relationship of IDPs, important both for understanding the bioc hemistry of membraneless organelles, as well as for designing biomaterials and synthetic organelles.
~ Coffee/tea will be served prior to lecture~
X-EXTRAINFO:Location CCB Auditorium (1303)\n\nHosted by Professor Sagar Khare DTSTAMP:20240328T191246 DTSTART:20190903T150000 DTEND:20190903T160000 SEQUENCE:0 TRANSP:OPAQUE END:VEVENT END:VCALENDAR